Treponema pallidum Particle Agglutination

TEST NAME Treponema pallidum Particle Agglutination (TP-PA)
  • This test is not approved for testing spinal fluids.
  • This test is performed as a reflex test . If requesting test as an add-on test, justification for testing must be included on the test order.


-Test Order Code: 86592

-Test Order Description: Syphilis Test

*Performed as reflex testing to Reactive RPR


TDH DLS Requistion:  PH-4182 Clinical Submission Requisition Form

Note: If requesting as add-on test, please include justification for testing. 

Specimen Requirements

Patient Preparation

  • None
Specimen Collection
  • Whole blood collected in Red Top Serum Coagulation Tube or Serum Separator Tube (SST).
  • Serum in sterile, plastic screw capped vial.
Specimen Labeling
  • Specimen should be labeled with at least two unique patient identifiers that match corresponding test order.
Specimen Processing
  • If testing will be delayed for more than seven (7) days,  aliquot and freeze serum at (-20)°C or colder.
Specimen Storage and Preservation
  • Specimens may be stored at 2 - 30°C for up to 72 hours post collection, or 2-8°C for up to seven (7) days. 
  • If testing will be delayed more than seven (7) days stored at 2 to 8°C, aliquot serum into a sterile, plastic, screw-capped vial and store at -20°C or colder.
  • Do not exceed more than one (1) freeze/thaw cycle.
Specimen Transportation
  • Ship specimens at 2 - 8°C on cold packs, if possible. Specimens may be shipped at 2-30°C if receipt at laboratory will occur within 72 hours post collection.  
  • Specimens should be be placed in a biohazard bag with absorbent material.
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180). 
Specimen Acceptability and Rejection
  • Grossly hemolyzed, lipemic, or microbially contaminated specimens will be rejected. 
  • If expiration date on collection tube is prior to collection date, specimen will be rejected. 
  • Specimens received after expiration of viability will be rejected. 
  • Unlabeled or improperly identified specimens will be rejected. 
  • Specimens collected in unapproved tube types will be rejected. 
  • Specimens broken in transit will be rejected. 
  • Specimens leaking in transit may be rejected after assessment of viability and contamination. 
  • Specimens with insufficient volumes of serum will be rejected. 
  • Specimens with clerical errors in the lab order or on the specimen tube will be rejected. 
  • Specimens transported or stored at incorrect temperatures will be rejected.
Testing Location
  • Nashville 
  • Knoxville

This Page Last Updated: April 11, 2024 at 11:39 AM