Syphilis Screen by RPR

TEST NAME Syphilis Screen by RPR
ALTERNATE NAME(S) Treponema pallidum, RPR
METHODOLOGY Arlington Scientific, Inc (ASI) Rapid Plasma Reagin (RPR) Card Test For Syphilis (nontreponemal flocculation test, qualitative/semi-quantitative)
SPECIAL INSTRUCTIONS This test is not approved for testing spinal fluids.


Test Order Code:     86592

Test Order Description:     Syphilis Test

TDH DLS Requistion:     PH-4182 Clinical Submission Requisition Form

Specimen Requirements

Patient Preparation

  • Venipuncture
Specimen Collection
  • Acceptable specimen types:

-  Whole blood in Red Top Serum Coagulation Tube or Serum Separator Tube (SST)

-  Serum in sterile, plastic, screw-capped vial



Specimen Labeling
  • Specimen should be labled with at least two unique patient identifiers and match accompanying test order.
Specimen Processing
  • Follow the tube manufacture's processing instructions for collection tubes.
Specimen Storage and Preservation
  • Specimens may be stored at 2 - 30°C for up to 72 hours post collection.  
  • Specimens may be stored at 2 - 8°C for up to seven (7) days.
  • If testing will be delayed more than seven (7) days, aliquot serum into a sterile, plastic, screw-capped vial and store at -20°C or colder.
Specimen Transportation
  • Ship specimens at 2 - 8°C on cold packs, if possible. Specimens may be shipped at 2-30°C if receipt at laboratory will occur within 72 hours of collection.
  • Specimens should be be placed in a biohazard bag with absorbent material.
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180).
Specimen Acceptability and Rejection
  • Grossly hemolyzed, lipemic, or microbially contaminated specimens will be rejected. 
  • If expiration date on collection tube is prior to collection date, specimen will be rejected. 
  • Specimens received after expiration of viability will be rejected. 
  • Unlabeled or improperly identified specimens will be rejected. 
  • Specimens collected in unapproved tube types will be rejected. 
  • Specimens broken in transit will be rejected. 
  • Specimens leaking in transit may be rejected after assessment of viability and contamination. 
  • Specimens with insufficient volumes of serum will be rejected. 
  • Specimens with clerical errors in the lab order or on the specimen tube will be rejected. 
  • Specimens transported or stored at incorrect temperatures will be rejected.
Testing Location
  • Nashville
  • Knoxville