Rickettsia PCR

TEST NAME Rickettsia PCR
DISEASE/DISORDER Rocky Mountain Spotted Fever
ALTERNATE NAME(S) Rickettsia rickettsii, Rickettsia prowazekii
METHODOLOGY PCR
SPECIAL INSTRUCTIONS
  • PRIOR CONSULTATION REQUIRED.
  • REQUESTED THROUGH CONSULTATION WITH EPIDEMIOLOGY ONLY (VBD.HEALTH@TN.GOV) OR 615-262-6356
ORDERING INSTRUCTIONS
  • TDH DLS Requisition PH-4182 Clinicial Submission Form AND
  • Travel history (domestic or international) to areas where RMSF is endemic must be documented on the requisition

Specimen Requirements


Patient Preparation

  • Submit specimens prior to treatment within 14 days of illness onset or within 48 hours of initial treatment.
Specimen Collection
  • At least 200ul of venous, whole blood preserved with EDTA or Acid Citrate Dextrose Solution A   (ACD-A)
Specimen Labeling
  • Specimen must be labeled with at least two unique patient identifiers and match accompanying PH-4182 Clinical Submission Form.
Specimen Processing
  • None
Specimen Storage and Preservation
  • Store specimens at 2 - 8°C prior to shipping.
Specimen Transportation
  • Ship specimes at 2 - 8°C within 48 hrs of collection.
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180). 
Specimen Acceptability and Rejection
  • Unsatisfactory specimens include, but may not be limited to:

-  The specimen was broken or leaked in transit

-  No specimen was in the container submitted

-  Improper specimen type submitted

-  Specimens received at improper temperature

  • Specimens received without documentation of consultation and travel history will not be tested.
Testing Location
  • Nashville

This Page Last Updated: April 11, 2024 at 12:21 PM