Fungal Culture

TEST NAME Fungal Culture
ALTERNATE NAME(S) Mycology Culture
METHODOLOGY Morphology (Macroscopic and Microscopic), MALDI-TOF,  Sequencing, Biochemical Methods
ORDERING INFORMATION TDH DLS Requisition:  PH-4182 Clinical Submission Requisition Form

Specimen Requirements

Patient Preparation

  • None
Specimen Collection
  • Isolate submission (growth on culture media):

-Culture isolate on Sabouraud's agar or other appropriate media (i.e. potato dextrose agar, potato flake agar, inhibitory mold agar, brain heart infusion, brain heart infusion with sheeps blood, brain heart infusion with chloramphenicol, brain heart Infusion with gentamicin, Sabouraud brain heart infusion with chloramphenicol and gentamicin, SDA Emmons, SDA Emmons with cycloheximide and chloramphenicol) in screw capped tube.

-Only pure cultures will be accepted

-Isolate should be submitted on primary media or subculture after growth is visible. 

  • Specimen submission:

-Acceptable specimen types include: Abscess, body fluids,  blood, bone marrow, CSF, skin, tissue and wounds.

-Collect specimen in a sterile, leak-proof container.

-Blood and bone marrow samples should be submitted in routine blood culture bottles. 

-Tissues should be placed in saline and submitted in a sterile leak-proof container. 

Specimen Labeling
  • Specimen must be labeled with at least two unique patient identifiers and source. 
  • Specimen labeling must match accompanying PH-4182 Clinical Submission Requisition.
Specimen Processing
  • None
Specimen Storage and Preservation
  • Store isolate at 15 - 25°C prior to shipping.
  • Acceptable specimen types should be stored at 2-8°C except for CSF. 
Specimen Transportation
  • Ship acceptable specimens at 2-8°C except for CSF (ship at 15-25°C). 
  • Ship isolates at 15-25°C.
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180). 
Specimen Acceptability and Rejection
  • Acceptable specimen types include: Abscess, body fluids,  blood, bone marrow, CSF, skin, tissue and wounds.
  • Isolates in pure culture. 
  • Unsatisfactory specimens include, but may not be limited to:

-  Specimens that have leaked or were broken in transit

- Media was liquified

- Specimen contained gross contamination

- Missing required information

- Frozen specimens

- Raw samples received 3 days post collection

Testing Location
  • Nashville

This Page Last Updated: April 11, 2024 at 12:18 PM