Hepatitis B

TEST NAME Hepatitis B 
ALTERNATE NAME(S) Hep B, HBV, HBsAg, HBsAb, anti-HB(s)
METHODOLOGY Chemiluminescent Microparticle Immunoassay (CMIA) on the Abbott Architect Analyzer 

Hepatitis B Panel includes HBV surface antibody, surface antigen, and total Core antibodies.


- Test Order Code: HEPB
- Test Order Description: Hepatitis B Pan

TDH DLS Requisition:  PH-4182 Clinical Submission Requisition

Specimen Requirements

Patient Preparation
  • Venipuncture
Specimen Collection
  • Acceptable Specimen Sources/Type(s)

 - Whole blood in serum separator tube (SST)

- Whole blood in serum coagulation tube (red top)

Specimen Labeling
  • Specimen must be labeled with at least two unique patient identifiers and match accompanying test order.
Specimen Processing
  • Specimens should be centrifuged 1837 RCF (g) for 12 minutes.
Specimen Storage and Preservation
  • Specimens may be stored on or off the clot, red blood cells, or separator gel for ≤ three (3) days for specimens stored 21 to 22°C or up to six (6) days for specimens stored at 2 to 8°C. 
  • If testing will be delayed more than six (6) days for specimens stored at 2 to 8°C, aliquot serum into a sterile, plastic, screw-capped vial and store at ≤ -20°C. 
  • Avoid more than three (3) freeze/thaw cycle
Specimen Transport
  • Ship specimens at 2 to 8°C on cold packs. 
  • Specimen must be placed in a biohazard bag with absorbent material and should be shipped to the laboratory on the day of collection, if possible. 
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180). 
Specimen Acceptability and Rejection
  • Serum specimens collected in serum coagulation (red top) tubes or serum separator tubes will be accepted.
  • Grossly hemolyzed, lipemic, or microbially contaminated specimens will be rejected.
  • Cadaveric specimens will be rejected.
  • Specimens with clerical errors in the lab order or on the specimen tube will be rejected.
  • Specimens broken in transit will be rejected. 
  • Specimens leaking in transit may be rejected after assessment of viability and contamination. 
  • Specimens with insufficient volumes of serum will be rejected. 
  • Specimens transported or stored at incorrect temperatures will be rejected.
  • Specimens received after expiration of viability will be rejected.
  • Unlabeled or improperly identified specimens will be rejected.
Testing Location
  • Nashville
  • Knoxville

This Page Last Updated: April 11, 2024 at 12:40 PM