Herpes Simplex Virus

TEST NAME Herpes Simplex Virus 1 & 2 detection by Nucleic Acid Amplification (NAAT)
DISEASE/DISORDER Herpes Simplex Virus 1 & 2
METHODOLOGY Aptima® Herpes Simplex Viruses 1 & 2 assay detection by nucleic acid amplification (NAAT) using the Hologic Panther System




Test Order Code: HSVPCR

Test Order Description: Herpes Simplex Virus PCR

TDH DLS Requisition:  PH-4182 Clinical Submission Requisition

Specimen Requirements

Patient Preparation
  • Refer to manufacturer (Hologic) instructions provided with collection kits for specific patient preparation information.
Specimen Collection
  • Refer to appropriate specimen collection kit package insert for collection instructions. 
  • Only the following specimen types will be accepted for testing:

- External cutaneous vesicle/lesion scrapings

- Anogenital swab

  • Specimens must be collected using the Aptima Multitest Swab Specimen Collection Kit (Orange tube)
  • Do not touch or mark upon the collection vial’s pierceable foil cap.
Specimen Labeling
  • Specimen must be labeled with at least two unique patient identifiers and specimen source and match accompanying test order.
Specimen Processing
  • After collection, place patient sample in biohazard bag with absorbent material using care not to touch the foil cap.
Specimen Storage and Preservation
  • Transport and store the specimen in the Aptima Multitest Swab Transport Media (STM) at 2°C to 30°C until tested.
  • Specimens must be tested with the Aptima HSV 1 & 2 assay within 36 days of collection. Specimens stored at -20°C to -70°C for up to 36 days may also be tested with the Aptima HSV 1 & 2 assay. 
  • Aptima swab specimens may be frozen and thawed up to 3 times prior to testing. 
Specimen Transport
  • Specimen must be placed in an individual biohazard bag with absorbent material and should be shipped to the laboratory on the day of collection for same day/next day receipt and processing.
  • Store and transport at 2 - 30°C; all specimens must arrive within 36 days of collection.
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180). 
Specimen Acceptability and Rejection
  • Specimens received in improper collection kits will be rejected. 
  • Specimens received that indicate invalid collection sites (e.g. throat, oral, mouth, tongue, mucous, rectal, ocular, nasal, endocervical, etc.) will be rejected.
  • Specimens received >36 days post-collection will be rejected.
  • If expiration date on collection tube is prior to collection date, specimen will be rejected. 
  • Specimens received with no swab, two swabs, a cleaning swab, and/or a swab not supplied by Hologic will be rejected.
  • Specimens with clerical errors in the lab order or on the specimen tube will be rejected. 
  • Unlabeled or improperly identified specimens will be rejected. 
  • Specimens transported or stored at incorrect temperatures will be rejected.
Testing Location
  • Nashville

This Page Last Updated: April 11, 2024 at 12:41 PM