Expanded Antimicrobial Susceptibility Testing

for Hard-to-Treat Infections

TEST NAME Expanded Antimicrobial Susceptibility Testing for Hard-to-Treat Infections
DISEASE/DISORDER Hard-to-Treat Enterobacterales infections
ALTERNATE NAME(S) None
METHODOLOGY Broth micro dilution
SPECIAL INSTRUCTIONS
  • Contact the laboratory for details before submission. 
  • Testing appropriate for:

-Enterobacterales isolates that  test non-susceptible to all beta-lactams, including either ceftazidime-avibactam or meropenem-vaborbactam. These isolates may be MBL-producing isolates with few effective treatment options.

-Enterobacteriaceae with NDM, VIM, or IMP genes confirmed by a molecular test.

ORDERING INFORMATION TDH ARLN Lab Web Portal

Specimen Requirements


Patient Preparation
  • None
Specimen Collection
  • Pure isolate on Non-selective media slant such as TSA, Blood, or Chocolate.
Specimen Labeling
  • Specimen must be labeled with at least two unique patient identifiers and match the accompanying TNDOH ARLN Lab Web Portal order. 
Specimen Processing
  • None
Specimen Storage and Preservation
  • Store specimens at ambient temperature until shipment. 
Specimen Transport
  • Ship specimens at ambient temperature. 
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180).
Specimen Acceptibility and Rejection
  • Only pure cultures will be accepted for testing.
  • Mixed cultures will be rejected.
  • Specimens that are mislabeled will be rejected.
  • Specimens that are broken in transit will be rejected.
  • Specimens received that do not meet the specimen requirements outlined in the Directory of Services or that deviate from the special instructions will not be acceptable for testing. 
Testing Location
  • Nashville

This Page Last Updated: September 15, 2025 at 12:14 PM