Hepatitis B Prenatal Screen

TEST NAME Hepatitis B Surface Antigen
DISEASE/DISORDER Hepatitis 
ALTERNATE NAME(S) HBsAg
METHODOLOGY Chemiluminescent Microparticle Immunoassay (CMIA) on the Abbott Architect Analyzer 
SPECIAL INSTRUCTIONS

Hepatitis B surface antigen testing.

ORDERING INFORMATION

Lab Web Portal

PTBMIS:

- Test Order Code Suggestion: HBsAgOB
- Test Name Suggestion: Hep B surface Ag Prenatal

Specimen Requirements


Patient Preparation
  • Venipuncture
Specimen Collection
  • Acceptable Specimen Sources/Type(s):
 - Whole blood in serum separator tube (SST)
Specimen Labeling
  • Specimen must be labeled with at least two unique patient identifiers and match accompanying test order.
Specimen Processing
  • Follow the tube manufacturer's processing instructions for collection tubes.
Specimen Storage and Preservation
  • Specimens may be stored on or off the clot, red blood cells, orseparator gel for:

- Up to 24 hours post collection at 2- 30°C or

- Up to six (6) days at 2 - 8 °C

  • If testing will be delayed more than six (6) days aliquot serum and store at (-20)°C or colder.
  • Avoid three (3) or more freeze/thaw cycles.
Specimen Transport
  • Ship specimens at 2 to 8°C on cold packs. 
  • Specimen must be placed in a biohazard bag with absorbent material and should be shipped to the laboratory on the day of collection, if possible. 
  • All infectious substance shipments must conform to U.S. Department of Transportation (DOT) Hazardous Materials Regulations (HMR 49 C.F.R. Parts 171-180). 
Specimen Acceptability and Rejection
  • Serum specimens collected in serum coagulation (red top) tubes or serum separator tubes will be accepted.
  • Grossly hemolyzed, lipemic, or microbially contaminated specimens will be rejected.
  • Cadaveric specimens will be rejected.
  • Specimens with clerical errors in the lab order or on the specimen tube will be rejected.
  • Specimens broken in transit will be rejected. 
  • Specimens leaking in transit may be rejected after assessment of viability and contamination. 
  • Specimens with insufficient volumes of serum will be rejected. 
  • Specimens transported or stored at incorrect temperatures will be rejected.
  • Specimens received after the expiration of viability will be rejected.
  • Unlabeled or improperly identified specimens will be rejected.
Testing Location
  • Nashville

This Page Last Updated: May 18, 2026 at 10:42 PM